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1-Step NBT/BCIP plus Suppressor Solution
Formulation: Proprietary solution of NBT, BCIP and levamisole
Sufficient For: 10 uses at 10mL each
Thermo Scientific Pierce NBT and BCIP Substrates are powders and ready-made solutions of nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate for chromogenic blot and IHC staining with alkaline phosphatase probes.
The combination of NBT (nitro-blue tetrazolium chloride) and BCIP (5-bromo-4-chloro-3'-indolyphosphate p-toluidine salt) yields an intense, insoluble black-purple precipitate when reacted with alkaline phosphatase, a popular enzyme conjugate for antibody probes. Pierce NBT and BCIP powders are available individually and in two convenient, ready-to-use solutions (with and without a levamisole suppressor of endogenous phosphatase activity).
Highlights:
AP substrate – for detection of alkaline phosphatase (alk-phos) activity on solid media, including nitrocellulose and PVDF membranes and fixed tissue samples
Chromogenic – no special equipment needed for visualization; produces purple-black precipitate that is easy to photograph
Package options – choose individual NBT and BCIP powders, or preformulated, ready-to-use solutions with or without levamisole suppressor
Highlights of Pierce NBT/BCIP 1-Step Solution (Part No. 34042):
Best for immunoblotting applications
Ready-to-use single component
Sensitive with low background and minimial assay-to-assay variability
Highlights of Pierce NBT/BCIP plus Suppressor 1-Step Solution (Part No. 34070):
Best for immunohistochemistry applications
Ready-to-use single component
Contains 1mM levamisole to inhibit endogenous phosphatase activity, while yielding an intense dark purple precipitate in the presence of calf intestinal phosphatase |