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Pierce 6xHis Protein Tag Staining Kit
Detect His-tagged proteins directly in gel using fluorescence.
The Thermo Scientific Pierce 6xHis Protein Tag Stain Kit is a fluorescent stain for preferential detection of histidine-tagged (His-tagged) recombinant fusion proteins in protein polyacrylamide gels.
The unique fluorescent stain specifically detects histidine-tagged fusion proteins that have been electrophoresed in polyacrylamide gels (e.g., SDS-PAGE). With this stain, expression of His-tagged recombinant proteins can be assessed directly in-gel, eliminating membrane transfer and Western blotting steps that are typically used to confirm purity and yield of tagged proteins. Although the lower limit of detection depends on which UV lamp (300nm) and detection equipment (CCD camera best) are used, the kit is convenient for in-process assessment of His-tagged protein production and is compatible with subsequent staining with coomassie dye and other total protein stains.
Highlights:
Two- to three-times faster than Western blotting – get results sooner and save hours of valuable research time
Detects directly on the gel – can eliminate the need for a membrane transfer and Western blot step for the detection of at least 0.2µg of 6xHis-tagged protein
Ready-to-use, two-reagent formula – no mixing, no diluting and no fuss; guarantees a simple-to-perform, mistake-free detection protocol
Specific fluorescent detection of 6xHis-tagged proteins – see only what you want to see (CCD camera recommended for low abundance proteins; UV-transilluminator for abundant proteins)
Compatible with double-staining – follow His-tagged protein staining with Thermo Scientific GelCode Blue Stain Reagent for a total protein profile determination
Includes:
Stain and developer solutions; Complete kit (Part No. 24575) also includes positive and negative control lysates (Part No. 24572)
Requires:
UV lamp source (300nm) and CCD camera |