NBT/BCIP Stock Solution ApplicationColorimetric AP substrate for: •Southern blot•Western blot•Immunohisto/cytochemistryProduct DescriptionFormulas: NBT: C40H30Cl2N10O6, BCIP: C8H6NO4BrClP x C7H9NMolecular weights: NBT: Mr = 817.7, BCIP toluidine salt: Mr = 433.6Reaction product:Color: blueSolubility in water: insolubleEvaluation: visually ContentsSolution of 18.75 mg/ml nitro blue tetrazolium chloride and 9.4 mg/ml 5-bromo-4-chloro-3-indolyl-phosphate, toluidine-salt in 67% (DMSO) (v/v)