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产品介绍:
异硫氰酸荧光素 (fluorescein isothiocyanate, FITC) FITC纯品为黄色或橙黄色结晶粉末，易溶于水和酒精溶剂。有两种异构体，其中异构体Ⅰ型在效率、稳定性与蛋白质结合力等方面都更优良。FITC分子量为389.4，最大吸收光波长为490～495nm，最大发射光波长为520～530nm，呈现明亮的黄绿色荧光。FITC在冷暗干燥处可保存多年，是目前应用最广泛的荧光素。其主要优点是人眼对黄绿色较为敏感，通常切片标本中的绿色荧光少于红色。Sigma相关产品资料：
Sigma F7250 Fluorescein isothiocyanate isomer I
suitable for protein labeling, ≥90% (HPLC), powder
Synonym: FITC, Fluorescein 5-isothiocyanate
CAS Number: 3326-32-7
Linear Formula: C21H11NO5S
Molecular Weight: 389.38
Beilstein Registry Number: 1407295
EC Number: 222-042-0
MDL number: MFCD00005063
PubChem Substance ID: 24894933
Description
Application Reagent for the FITC labeling of proteins; Microsequencing of proteins and peptides (HPLC)
Biological applications include use as a fluorescent labeling reagent for proteins, a fluorescent reagent for protein tracing, and a reagent in the fluorescent antibody technique for the rapid identification of pathogens. FITC is yellow-orange in color with an absorption maximum at 495 nm. Upon excitation it emits a yellow-green color with an emission maximum at 525 nm.
Properties
assay ≥90% (HPLC)
form powder
fluorescence λex 492 nm; λem 518 nm (green)
mp >360 °C(lit.)
suitability suitable for protein labeling
storage temp. 2-8°C
Safety
Personal Protective Equi1.aspx">pment dust mask type N95 (US), Eyeshields, F1.aspx">aceshields, Gloves
Hazard Codes Xn
Risk Statements 42/43
Safety Statements 22-36/37-45
WGK Germany 3 |