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Ficoll PM400 (Dry Powder)
Application: Used in the formation of density gradients for the separation and isolation of eukaryotic cells, organelles, and bacterial cells, as a stabilizing agent and as a separation medium for the isolation of lymphocytes.
It also has applications in defined culture media, nucleic acid hybridization, electrophoresis, and immunological studies.
Used for gradient centrifugation in all types of centrifuge rotors and for separation at unit gravity.
Density Max.: 1.2 g/ml
Endotoxin Activity Max. : 0.12 EU/mg
Estimated Shelf Life from Manufacture Date: 5 years
Storage Conditions: 4 - 30°C
Stability: Stable in alkaline and neutral solutions, but are rapidly hydrolyzed in solution at pH 3, especially at elevated temperature
Physical State: Solid
Physical Form: Spray-dried powder
Molecular Weight [Mr] Max.: ~ 5 x 10~ 5
Molecular Weight [Mr] Min.: ~ 3 x 105
Reactivity and Stability: The reactivity and stability of Ficoll PM400 are determined by its hydroxyl groups and the glycosidic bonds in the sucrose residues. It is stable in alkaline and neutral solutions. At pH values lower than 3, it is rapidly hydrolyzed, especially at elevated temperatures. In neutral solutions, however, Ficoll PM 400 can be sterilized by autoclaving at 110°C for 30 min without degradation. Strong oxidizing and reducing agents should be avoided.
Stokes Radius: ~ 10 nm
Color: White
Specific Rotation [alpha]20: 50°-65°
Intrinsic Viscosity: ~ 17 ml/g
Solubility: It is delivered as a spray-dried powder and is therefore readily soluble in aqueous media when it is added slowly with concomitant stirring. Any lumps formed during this procedure will dissolve fairly quickly. Concentrations up to 50% (w/v) can easily be attained |