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Glutathione Sepharose 4 Fast Flow
Ligand: Glutathione and 10-carbon linker arm
Average Particle Size: 90 µm
Matrix: Highly cross-linked 4% agarose
Binding Capacity/ml Chromatography Medium: >10 mg/ml1)
pH Stability Working Range: 3-12
pH Stability Cleaning-in-Place (CIP): 3-12
Pressure/Flow Specification: base matrix 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm
Flow Velocity: <450 cm/h
Storage Conditions: 4 to 30°C, 20% Ethanol
Chemical Stability: All commonly used aqueous buffers, e.g. 1 M acetate, pH 6.0, 0.1 M NaOH, 70% ethanol or 6 M guanidine-hydrochloride for 1 h at room temperature
1)Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.
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