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Butyl Sepharose™ 4B
Butyl Sepharose 4B is a separation media for hydrophobic interaction
chromatography (HIC). Substances are separated on the basis of their
different hydrophobicities.
The butyl group is coupled to 4% agarose matrix by the cyanogen
bromide method.
Ligand density : 10 to14 μmol butyl groups/ml drained
medium
Available capacity: Application dependent
Matrix: 4% agarose
Particle size range: 45 to 165 μm
Mean particle size 90 μm
Maximum flow velocity
75 cm/h at 25ºC, HR 16/10 column,
5 cm bed height
pH stability2
- short term:4 to 8
- long term :4 to 8
Chemical stability: Stable to all commonly used aqueous
buffers
Physical stability: Negligible volume variation due to
changes in pH or ionic strength
Autoclaving: Not recommended |