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Lyticase from Arthrobacter luteus
溶壁酶Lyticase/Lysing Enzymes
厂家：sigma
货号：L2524
规格：100MG
CAS号 37340-57-1
保存温度：-20°C
Application
Yeast cells are difficult to disrupt because the cell walls may form capsules or resistant spores. DNA can be extracted from yeast by using lysing enzymes such as lyticase, chitinase, zymolase, and gluculase to induce partial spheroplast formation; spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest cell walls of yeast and generate spheroplasts from fungi for transformation.
Reported to be useful for lysis of Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloeckera, Kluyveromyces, Lipomyces, Metschikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomycopsis, Saccharomycodes, and Schwanniomyces species.
Unit Definition
One unit will produce a ΔA800 of 0.001 per min at pH 7.5 at 25 °C, using a suspension of yeast as substrate in a 3 mL reaction mixture.
Physical form
Partially purified, lyophilized powder containing potassium phosphate buffer salts and stabilizers
form lyophilized powder
composition Protein, ≥20% biuret |