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产品介绍:
Hoechst 33258，也称bisBenzimide H 33258或HOE 33258。分子式为C25H24N6O•3HCl，分子量为533.88，CAS Number 23491-45-4。
Hoechst 33258是一种可以穿透细胞膜的蓝色荧光染料，对细胞的毒性较低。
Hoechst 33258染色常用于细胞凋亡检测，染色后用荧光显微镜观察或流式细胞仪检测。Hoechst 33258也常用于普通的细胞核染色，或常规的DNA染色。
Hoechst 33258的最大激发波长为346nm，最大发射波长为460nm；Hoechst 33258和双链DNA结合后，最大激发波长为352nm，最大发射波长为461nm。
溶解度：Hoechst 33258溶于水，溶解度可达10mg/ml。
用途：用于细胞核染色时，推荐的Hoechst 33258工作浓度为0.5-10μg/ml。
Sigma相关资料：
Sigma-Aldrich B2883 bisBenzimide H 33258
≥98% (HPLC and TLC)
Synonym: BBIH, HOE 33258, Hoechst 33258, 2′-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi(1H-benzimidazole) trihydrochloride, 2-[2-(4-Hydroxyphenyl)-6-benzimidazoyl]-6-(1-methyl-4-piperazyl)benzimidazole trihydrochloride
CAS Number: 23491-45-4
Linear Formula: C25H24N6O • 3HCl
Molecular Weight: 533.88
Beilstein Registry Number: 4088183
EC Number: 245-690-6
MDL number: MFCD00012679
PubChem Substance ID: 24891414
Description
Biochem/physiol Actions Membrane-permeable, fluorescent DNA stain with low cytotoxicity that intercalate in A-T regions of DNA.
Application Useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry.
Excitation max. = 346 nm
Emission max. = 460 nm
Properties
assay ≥98% (HPLC and TLC)
solubility H2O: 10 mg/mL
phosphate buffers: precipitates
suitability passes application test for fluorescence
storage temp. −20°C
Safety
Personal Protective Equipment dust mask type N95 (US), Eyeshields, Gloves
Hazard Codes Xn
Risk Statements 22-36/38
Safety Statements 26-36
WGK Germany 3
RTECS SM1140500 |