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猪胰腺弹性酶是一种丝氨酸蛋白酶，它能水解氨基化合物和酯。弹性酶的特殊之处在于它能作用于弹性蛋白。酶作用的最佳pH值为8.5。大豆胰蛋白酶抑制剂和激肽释放酶抑制剂能抑制其蛋白水解活性，但不能抑制其弹性蛋白水解活性。
弹性蛋白酶是从猪胰腺中分离提取的，并进一步用层析的方法制备，以去除残留的其它蛋白酶的污染。
Sigma相关产品资料：
Sigma E1250 Elastase, pancreatic from porcine pancreas
Type I, aqueous suspension, ≥4.0 units/mg protein
Synonym: Elastase from hog pancreas, Pancreatopeptidase E
CAS Number: 39445-21-1
Enzyme Commission (EC) Number: 3.4.21.36 ( BRENDA | IUBMB )
EC Number: 254-453-6
MDL number: MFCD00130998
Description
Packaging Package size based on protein content
Preparation Note 2× crystallized
Unit Definition One unit will hydrolyze 1.0 μmole of N-succinyl-L-Ala-Ala-Ala-p-nitroanilide per min, pH 8.0 at 25 °C.
Properties
type Type I
form aqueous suspension
contains ~0.01% thymol
foreign activity trypsin ≤50 BAEE units/mg protein
storage temp. 2-8°C
Safety
Personal Protective Equipment dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Hazard Codes Xn
Risk Statements 36/37/38-42
Safety Statements 23-24-26-36/37
WGK Germany 1 |