|
由玫瑰链霉菌Streptomyces roseus MB-26-AI产生的胰蛋白酶抑剂，它是许多蛋白酶抑制剂中的一种。纯亮肽素熔点110～140℃，旋光度-56°(c=1.0，甲醇)。溶于水、甲醇、乙醇、丁醇、二甲亚砜，不溶于乙酸乙酯、丙酮、氯仿、己烷等，对Rydon-Smith、坂口呈附哇反应，对茚三酮、三氯化铁、蒽酮、莫利氏呈阳性反应。亮肽素可抑制胰蛋白酶、木瓜蛋白酶、组织蛋酶B、血纤维蛋白溶酶、激肽释放酶等，它的抑制作用与底物呈竞争抑制。亮肽素（Leupeptin）能抑制多种丝氨酸和半胱氨酸蛋白酶；抑肽素（pepstatin）抑制天冬氨酸蛋白酶；抑肽酶（aprotinin）能抑制许多丝氨酸蛋白酶；苯丁抑制剂（bestatin）能抑制氨基酸多肽酶 .
别名：亮肽素
分子式：C20H38N6O4 1/2(H2SO4) H20 分子量： 493.62
CAS＃：103476-89-7
外观：白色粉末
特性：比旋度(2%, 水)：-84.2--82.2
溶解性：溶于水，参考浓度为10mg/ml，工作浓度为 0.5mg/ml。
储存液4℃保存一周，-20℃保存6个月。
Sigma相关产品信息
Sigma L2884 Leupeptin hemisulfate salt
microbial, ≥90% (HPLC)
Synonym: Acetyl-Leu-Leu-Arg-al, N-Acetyl-L-leucyl-L-leucyl-L-argininal hemisulfate salt
CAS Number: 103476-89-7
Linear Formula: C20H38N6O4 • 1/2H2SO4
Molecular Weight: 475.59
MDL number: MFCD00037012
PubChem Substance ID: 24896328
Description
Analysis Note Leupeptin gives multiple peaks on HPLC due to equilibria among three forms in solution. Purity determined using three main peaks. Majority of contaminating peptide is racemized leupeptin.
Biochem/physiol Actions Inhibitor of serine and cysteine proteases. Inhibits plasmin, trypsin, papain and cathepsin B. No inhibition found with pepsin, cathepsins A and D, thrombin, or α-chymotrypsin. Effective concentration 10-100 μM.
性质
biological source microbial
assay ≥90% (HPLC)
EQP level Premium
solubility H2O: 10 mM (Solutions are stable for a week at 4 °C. Stock solutions are stable up to 6 months at −20 °C.)
storage temp. −20°C |