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中文名：核糖核酸酶I,核糖核酸酶A
英文名：Ribonuclease,bovine pancrease,Ribonuclease I,Ribonuclease A,Pancreatic ribonuclease,Ribonucleate pyrimidinenucleotide-2′-transferase,Ribonucleate 3′-pyrimidino-oligonucleotidohydrolase,Rnase I,Rnase,Rnase A
分子量：相对分子质量13000～15000
性状：一种水解核糖核酸的酶。商品为白色结晶或冻干粉末,也有50%甘油的稠状溶液。对湿敏感。极易溶于水。酶反应：专一地催化核糖核酸的核糖部分3′和5′磷酸二酯键的裂开,形成具有2′,3′-环一磷酸衍生物寡聚核苷酸。水溶液在25℃以下相当稳定,最大稳定范围pH 2～4.5。最适pH 7.7。最适温度65℃。等电点(pI)7.8。比旋光度 [α]25D-0.47°(以每mG氮计,c=5,水中)。抑制剂有0.0005mol/L镁(MG2+)、脱氧核糖核酸(竞争性抑制剂)、变性脱氧核糖核酸(比天然的更有效)。
用途：生化研究,测定核酸的结构,脱氧核糖核酸和核糖核酸的降解。核糖核酸酶 A （Ribonuclease A）来源于牛胰，为高度专一性核酸内切酶，可特异攻击 RNA 上嘧啶核苷酸的C′3上的磷酸根和相邻核苷酸的C′5之间的键，产物为3′嘧啶单核苷酸或以3′嘧啶核苷酸结尾的低聚核苷酸。可除去 DNA:RNA或RNA:RNA杂合体中未杂交的 RNA 区;可用来确定 DNA 或 RNA 中单碱基突变的位置, 用标记了的RNA探针与待检含单碱基置换的DNA或RNA退火,RNA：RNA或RNA：DNA中的错配碱基可被RNaseA切割。广泛用来去除 DNA 样品中的 RNA 。
Sigma相关产品资料：
Sigma R4875 Ribonuclease A from bovine pancreas
Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein
Synonym: Pancreatic Ribonuclease, Ribonuclease I, Ribonucleate 3′-pyrimidinooligonucleotidohydrolase, RNase A
CAS Number: 9001-99-4
Enzyme Commission (EC) Number: 3.1.27.5 ( BRENDA | IUBMB )
EC Number: 232-646-6
MDL number: MFCD00132181
Description
Analysis Note Protein determined by E1%/280.
Application • RNase protection assays
• Remove unspecifically bound RNA
• Analysis of RNA sequences
• Hydrolyze RNA contained in protein samples
• Purification of DNA
Pancreatic RNase A specifically cleaves at the 3′-side of pyrimidine (uracil or cytosine) phosphate bonds. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can also hydrolyze RNA from protein samples.
Preparation Note Salt fractionated and chromatographically purified.
性质
type Type I-A
assay ≥60% RNase A basis (SDS-PAGE)
form powder
mol wt mol wt ~13,700
total impurities salt, essentially free
foreign activity protease, essentially free
storage temp. −20°C
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