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Ficoll-Paque PREMIUM密度为1.077 g/ml，经过优化用于从人 外周血分离单核细胞，所采用的方法为由Boyum (3) 改进的 简便快速的离心方法。此介质亦可用于从包括骨髓和脐带血 等组织来源中分离人单核细胞。采用推荐的方法分离人外周 血获得的单核细胞悬液具有以下特点： ● 分离所得的细胞中95 ± 5%为单核细胞 ● 分离所得的细胞存活细胞率 > 90% ● 可回收原始的血液样本中60 ± 20%的单核细胞 ● 3 ± 2%的粒细胞 ● 5 ± 2%的红细胞 Application: Optimized for the isolation of mononuclear cells from human peripheral blood, bone marrow, and umbilical cord blood.
Endotoxin Activity Max.: < 0.12 EU/ml Properties: Ficoll-Paque PREMIUM products constitute a range of sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA in water for injection (WFI). The densities and osmolalities of the different products have been optimized for the isolation of mononuclear cells from blood and bone marrow Estimated Shelf Life from Manufacture Date: 3 years Storage Conditions: 4–30°C Stability: Ficoll-Paque PREMIUM products are provided as sterile solutions which are stable for at least 3 yr when stored between 4°C and 30°C and protected from light. Deterioration of Ficoll-Paque PREMIUM products is indicated by the appearance of a yellow color or particulate material in the solution Physical State: Liquid Osmolality Max: 310 mOsm/kg pH Range: 5.5–7.5 Color: Colorless to slightly yellow Certificate of Analysis: Yes
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