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Recombinant Human FGF-acidic
Catalog Number: 100-17A
Synonyms: Fibroblast Growth Factor-acidic, FGF-1, HBGF-1, ECGF-beta
Description: FGF-acidic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-acidic is a non-glycosylated heparin binding growth factor that is expressed in the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes and endothelial cells. FGF-acidic has the ability to signal through all the FGF receptors. Recombinant human FGF-acidic is a 16.0 kDa protein consisting of 141 amino acid residues.
AA Sequence: MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D
Source: E.coli
Purity: Greater than 95% by SDS-PAGE gel and HPLC analyses.
Biological Activity: Assay #1: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is ≤ 10 ng/ml, corresponding to a specific activity of ≥ 1 x 105 units/mg.
Assay #2: The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is ≤ 0.5 ng/ml in the presence of 10 μg/ml heparin, corresponding to a specific activity of ≥ 2 x 106 units/mg.
Cross Reactivity: Cow, Mouse, Pig, Rat, Shark, Squid, Human, Hamster, Hagfish, Leech, N/A
Country of Origin: USA |