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PCNA ELISA Kit
Fast, sensitive assay for the in vitro quantitation of PCNA protein. The kit has broad reactivity, and is suitable to detect PCNA in autoimmune sera samples. The assay was validated using the BrdU Cell Proliferation Assay (Cat. No. QIA58). Proliferating cell nuclear antigen (PCNA) has no known enzymatic activity; however, it can interact with a variety of proteins and regulate their activities. These varied interactions with DNA metabolic proteins imply that PCNA is a central factor for the coordination of DNA replication, DNA repair, epigenetic inheritance, and cell-cycle control.
产品信息
Format 96-well strip plate
Form 96 Tests
Detection method Colorimetric
Species reactivity human, mouse, rat
Do not freeze 是的
Unit definition One unit (U) is defined as the amount of PCNA measured from ~ 2.6 x 103 SW620 cells.
Specificity Human PCNA protein
Assay range 3.125-200 ng/ml of PCNA
Assay time 3.5 h
Sample type Cell lysates, tissue culture media , serum, or plasma
Kit contains 96-Well Removable Strip Plate, 2 vials of PCNA Standard, Detector Antibody, 400X Conjugate, Conjugate Diluent, Substrate, Sample Diluent, 20X Plate Wash Concentrate, Antigen Extraction Agent, Stop Solution, Plate Sealers, and a user protocol.
商店和运输信息
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage -20°C
Ship Dry Ice Only
Multiple Toxicity Values, refer to MSDS |