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QIA39 FragEL DNA Fragmentation Detection Kit, Fluorescent - TdT Enzyme
TUNEL Assay
Allows for detection of apoptosis at the individual cell level. Apoptosis morphology is easily detected. Detection is by flow cytometry or fluorescence microscopy. The kit mounting media permits the observance of the total cell population. Cat. No. QIA39 is a fluorescein-conjugated version of our widely referenced TdT Colorimetric FragEL™ DNA Fragmentation Detection Kit (Cat. No. QIA33) for non-isotopic labeling. Terminal deoxynucleotidyl transferase (TdT) binds to exposed 3′-OH ends of DNA fragments generated in response to apoptotic signals and catalyzes the addition of fluorescein-labeled and unlabeled deoxynucleotides. When excited, fluorescein generates an intense signal which can be detected by fluorescence microscopy or flow cytometry. The mounting media sustains the fluorescent signal from samples labeled on slides and, therefore, aids in the morphological evaluation and characterization of normal and apoptotic cells. Non-apoptotic cells can be visualized by a DAPI filter.
产品信息
Format Flow cytometry or fluorescence microscopy
Form 50 Tests
Detection method Fluorescence
Species reactivity a broad range of species
Applications Flow Cytometry
Avoid freeze/thaw 是的
Assay time 2 h
Sample type Frozen or paraffin sections, fixed cell preparations or cell suspensions
Kit contains Proteinase K, Equilibration and Labeling Buffers, TdT Enzyme (Cat. No. PF060), Control Slides, Mounting Media (Cat. No. HC08), and a user protocol.
商店和运输信息
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage -20°C
Ship Dry Ice Only
Multiple Toxicity Values, refer to MSDS
安全标示
S Phrase S: 26-27-36/37/39-45-53
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Take off immediately all contaminated clothing.
Wear suitable protective clothing, gloves and eye/face protection.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Avoid exposure - obtain special instructions before use.
R Phrase R: 23/25-34-36/37/38-42/43-45-61
Toxic by inhalation and if swallowed.
Causes burns.
Irritating to eyes, respiratory system and skin.
May cause sensitization by inhalation and skin contact.
May cause cancer.
May cause harm to the unborn child. |