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MMP-9, Proenzyme, Human, Recombinant, CHO Cells
92 kDa Gelatinase, Matrix Metalloproteinase 9, Gelatinase B, Type IV Collagenase
Recombinant, human pro-MMP-9 expressed in CHO cells. A portion (less than 10%) of the enzyme may be seen as a naturally-occurring dimer. Dimerization does not interfere with activation. May contain up to 10% TIMP proteins. During storage, a small portion (less than 10%) of the enzyme may also become activated. Requires activation prior to use. A simple activation protocol is included.
产品信息
Form Liquid
Formulation In 150 mM NaCl, 50 mM Tris-HCl, 10 mM CaCl2, 0.05% BRIJ®-35 Detergent, pH 7.5.
Preservative None
Molar mass 92000
Applications
Immunoblotting (Western Blotting)
Substrate Cleavage Assay
Zymography
Avoid freeze/thaw 是的
Unit definition Specific activity is determined using 10 μM (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-(3-[2, 4-dinitrophenyl]-L-2, 3-diaminopropionyl)-Ala-Arg-NH2 (excitation 320 nm, emission 405 nm), and 20 ng enzyme in 100 μl of 50 mM Tris-HCl, pH 7.5, 10 mM CaCl2, 150 mM NaCl, and 0.05% BRIJ®-35 Detergent at room temperature.
Activity >1,300 pmoles/min/µg
Purity >90% by SDS-PAGE
EC number 3.4.24.35
商店和运输信息
Storage ≤ -70°C
Ship Dry Ice Only
Standard Handling |