PhosphoDetect™ Hsp27 (pSer78/82) ELISA Kit
Detects and quantifies the level of phosphorylated Hsp27 protein in cell lysates, tissue extracts, and biological fluids. Hsp27 is the small heat shock protein that acts as a molecular chaperone interacting with a large number of proteins. Hsp27 is known for its ability to negatively regulate the mitochondrial pathway to caspase activation. The phosphorylated form of Hsp27 also plays a major role in apoptosis through its ablity to prevent cell death by maintaining redox homeostasis and mitochondrial stability.
产品信息
Format 96-well plate
Form 96 Tests
Detection method Colorimetric
Assay range 0.1-10 ng/ml
Assay time 4.5 h
Sample type Cell lysates
Positive control Phosphorylated Hsp27 Standard, UV-treated HeLa or MCF7 cells or sorbitol-treated DU145 cells
Kit contains Hsp27-Coated Plate, Rabbit Anti-Hsp27 Detector Antibody, Phosphorylated Hsp27 Standard, HRP-Conjugate, Assay Diluent, ELISA 20X Plate Wash Concentrate, TMB Substrate, ELISA Stop Solution, and a user protocol
商店和运输信息
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage -20°C
Ship Blue Ice Only
Regulatory Review |