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A convenient kit for the identification of changes in the plasma membrane that occur during apoptosis. In apoptotic cells, phosphoatidylserine (PS) is translocated from the inner to the outer leaflet of the plasma membrane, allowing for the detection of PS on the cell surface. In the presence of Ca 2+, Annexin V exhibits a high affinity for PS and binds cells with exposed PS. Annexin V, when conjugated to a fluorophore, can be used to monitor apoptopic cells using flow cytometry or immunofluorescence microscopy.
Format Flow cytometry or fluorescence microscopy
Form 20 Tests
Detection method Fluorescence
Applications Immunofluorescence
Flow Cytometry
Assay time 30 min
Sample type Intact cells
Kit contains Annexin V-FITC, Binding Buffer, Propidium Iodide and a user protocol.
CBA059 Annexin V-FITC Apoptosis Detection Kit II
样本类型：完整细胞，有阳性对照，反应次数为20次，用于检测早期凋亡的细胞膜改变 |