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InnoZyme™ Calpain 1/2 Activity Assay Kit
A highly sensitive and specific assay for measuring Calpain 1 and 2 activity in biological samples like cell lysate or tissue extracts and for screening enzyme inhibitors. Calpains (calcium ion-dependent papain-like cysteine proteases) are widely distributed in all mammalian cells. They mediate calcium-induced activation of the Erk1/Erk2 MAPK pathway and are believed to participate in intracellular signal processing via limited proteolysis of their targets.
• Sensitive: detects calpain activity at 63-1000 ng/ml
• Specific: Substrate and inhibitor optimized for specific detection of calpain activity
• Versatile: Homogeneous 96-well format can be used with biological samples or for inhibitor screens
• Convenient: Contains all reagents to assay samples in 30 min
产品信息
Format 96-well plate
Form 96 Tests
Detection method Fluorometric
Assay range 63-1000 ng/ml as measured with purified human calpain 1.
Sample type Cell lysates, tissues extracts
Positive control Calpain 1
Kit contains 96-Well Plate, Calpain 1 Control, Substrate, Assay Buffer, Activation Buffer, Inhibitor, Reducing Agent, Plate Sealer and a user protocol.
商店和运输信息
Storage ≤ -70°C
Ship Dry Ice Only
Irritant |