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InnoZyme™ TACE Activity Kit
ADAM17 Activity Kit, α-Secretase Activity Kit, TNF-α Converting Enzyme Activity Kit
The InnoZyme™ TACE Activity Kit is a specific and sensitive assay designed to measure human TACE activity in cell lysates and biological samples and for screening enzyme inhibitors. An Anti-Human TACE-Coated 96-Well Plate is pre-coated with a monoclonal antibody specific for human TACE that captures the enzyme. Unbound material is discarded, the plate is washed, and the activity of captured TACE is measured using an internally quenched fluorescent substrate, MCA-KPLGL-Dpa-AR-NH2. Cleavage of the scissile amide bond, G-L, releases the fluorophore from the quenching molecule, Dpa, resulting in an increase in fluorescence. Fluorescence of the cleaved product, MCA-KPLG, is measured at an excitation wavelength of ~324 nm and emission wavelength of ~400 nm. The level of fluorescence is directly related to the enzyme activity.
• Highly specific: based on immunocapture of human TACE
• Highly sensitive and quantitative: assay range 5-100 ng/ml
• Convenient: 96-well format and non-radioactive detection
• Versatile: appropriate for measuring active TACE and high-throughput screening of TACE inhibitors
产品信息
Format 96-well plate
Form 96 Tests
Detection method Fluorometric
Protect from Light 是的
Avoid freeze/thaw 是的
Components
anti-human TACE-coated 96-well plate, TACE control, substrate, sample buffer, assay buffer, wash buffer, plate sealer, user protocol
Assay range 5-100 ng/ml as measured with purified recombinant human TACE
Sample type Cells
Positive control Human recombinant TACE
Kit contains Anti-Human TACE-Coated 96-Well Plate, Control, Substrate, Sample Buffer, Assay Buffer, Wash Buffer, Plate Sealer, and a user protocol.
Declaration Not available for sale in Germany.
商店和运输信息
Storage Multiple storage requirements
Ship Dry Ice Only
Multiple Toxicity Values, refer to MSDS |