InnoCyte™ ECM Cell Adhesion Assay, Laminin/Basement Membrane Complex (BMC)
This product has been discontinued.
The InnoCyte™ Cell Adhesion Assay, Laminin I/Basement Membrane Complex is designed for the determination of the relative attachment of adherent cell lines to laminin I and basement membrane protein complex, for evaluation of cell adhesion receptors, and for assessment of inhibitory or stimulatory substances for cell attachment. The kit is supplied with a 96-well strip plate coated with mouse laminin I and basement membrane complex (see plate format below). Cells are seeded in the coated wells and incubated at +37°C. Following incubation, the wells are washed briefly and attached cells are labeled the green fluorescent dye, calcein-AM. BSA-coated wells serve as a negative control and poly-L-lysine-coated wells serve as a positive control for general attachment. Relative cell attachment is assessed using a fluorescence plate reader.
• Convenient: 96-well plate format; allows cell attachment profiling of various cell lines at the same time
• Sensitive: cell-permeable fluorescent dye; no cell permeabilization needed
• Easy to use: does not require tedious staining method or expensive complicated equipment
产品信息
Format 96-well plate
Form 72 Tests (36 Laminin and 36 BMC)
Detection method Fluorometric
Protect from Light 是的
Avoid freeze/thaw 是的
Assay time 2.5 h
Sample type Adherent cultured cells
Kit contains Coated 96-Well Plates, Fluorescent Dye, D-PBS, and a user protocol.
商店和运输信息
Storage +2°C to +8°C
Ship Blue Ice Only
Multiple Toxicity Values, refer to MSDS |