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iFOLD® Protein Refolding System 3
This product has been discontinued.
The iFOLD® Protein Refolding System 3 is designed to screen for optimal refolding conditions for proteins expressed as inclusion bodies. The iFOLD System 3 utilizes FoldACE™ Reagents, which are novel, detergent-free refolding additives that have been demonstrated to significantly enhance refolding of certain proteins. The kit provides a systematic evaluation of 96 conditions representing different buffering systems, pH values, redox agents, and 5 different FoldACE Reagents. Following cell lysis with the aid of Lysonase™ Bioprocessing Reagent, membrane components and contaminating proteins trapped within inclusion body pellets are removed by a series of washes with Triton X-100. The purified inclusion bodies are reduced and denatured by addition of Tris(2-carboxyethyl) phosphine (TCEP) and guanidine hydrochloride and refolded by rapid dilution into the iFOLD System refolding buffers. Components for cell lysis, inclusion body washes and denaturation, and a pre-dispensed 96-well plate-based matrix of refolding buffers are included in the kit. Each kit is sufficient for screening up to 96 refolding conditions (91 experimental and 5 buffer-only control wells) for a single protein.
产品信息
Declaration Patent pending.
商店和运输信息
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage Multiple storage requirements
Ship Multiple Storage Conditions
Corrosive
安全标示
S Phrase S: 26-27-36/37/39
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Take off immediately all contaminated clothing.
Wear suitable protective clothing, gloves and eye/face protection.
R Phrase R: 34
Causes burns. |