|
Strep•Tag® II Perfect Protein™ Markers, 16–100 kDa, Lyophilized
Accurate, convenient molecular weight determination by SDS-PAGE and immunblotting
The Perfect Protein™ Markers are a novel set of recombinant proteins with defined sizes at convenient intervals. Designed for routine use in SDS-polyacrylamide gel electrophoresis, the Perfect Protein Markers enable highly accurate size determination of unknown samples. Unlike many conventional markers (e.g., ovalbumin, serum albumin, etc.), the Perfect Protein Markers contain no oligosaccharides that cause anomalous migration, heterogeneous "fuzzy" bands, or inaccurate size estimation. The markers are optimized for use with Coomassie blue staining, but adjusted amounts can also be used with other gel staining methods (e.g., silver staining, fluorescent dyes, etc.).
The Strep•Tag® II Perfect Protein™ markers, 16-100kDA include protein sizes of 16, 23.5, 30, 45 60, and 100 kDA. Each marker contains the Strep•Tag II sequence and can be detected by the Strep•Tag II Monoclonal Antibody (Cat. No. 71590) in Western blots. They are provided lyophilized and are supplied with a Strep•Tag II Marker Reconstitution Buffer which also serves as the loading buffer.
The 4X SDS Sample Buffer (Cat. No. 70607), which is separately available, is a standard formulation commonly used for SDS-PAGE analysis of proteins. The solution includes DTT for complete denaturation of disulfide bonds. The buffer can be used at a final concentration of 2X for most applications.
相关产品
69079 Perfect Protein™ Markers, 10-225 kDa
69149 Perfect Protein™ Markers, 15-150 kDa
产品信息
Features and benefits • Markers are supplied at the working dilution in gel loading buffer; concentration optimized for Coomassie blue staining
• Recombinant, unmodified markers produce sharp, accurately sized bands
商店和运输信息
Storage +2°C to +8°C
Ship Blue Ice Only
Standard Handling |