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pTriEx™-6 3C/LIC Vector Kit
Directional PCR cloning into a multisystem expression vector
The pTriEx™-6 3C/LIC vector is a 3C/LIC version of pTriEx-6. The pTriEx-6 3C/LIC vector is uniquely designed to allow rapid characterization of target genes in multiple expression systems. With this vector, a single recombinant plasmid can be used to test expression in E. coli, insect, and mammalian cells. Transient mammalian expression is mediated by the CMV immediate early enhancer and promoter. For expression in insect cells, the pTriEx-6 3C/LIC vector contains flanking baculovirus sequences to permit the generation of recombinant baculoviruses using the BacVector® System or BacMagic™ Kits. In baculovirus-infected insect cells, expression is driven by the very late p10 promoter. Expression in E. coli is regulated by the tightly controlled T7lac promoter. The pTriEx-6 3C/LIC vector contains an N-terminal 8 aa Strep•Tag® II coding sequence followed by a HRV 3C protease cleavage site and a multiple cloning site, with the option to encode optional sequences for a C-terminal thrombin cleavage site and a His•Tag® (10-histidine) fusion.
* Strep•Tag® technology for protein purification and detection is covered under US Patent No. 5,506,121, UK Patent No. 2,272,698 and French Patent No. 93 13 066. Strep•Tactin® is covered by US Patent No. 6,103,493. These products are provided only for research use. Information about licenses for commercial use is available from IBA GmbH, Rudolf-Wissell-Str. 28, D-37079 Goettingen, Germany.
The pTriEx vector is covered by US Patent 6,589,783 owned by EMD Millipore Corporation or its Affiliates. This Product is sold for research use only. Any commercial use of the Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Further, the pTriEx vector allows for expression of target genes under control of the T7 promoter and is also covered by US Patent 5,693,489. This patent is licensed by EMD Millipore Corporation from Brookhaven National Labs for research use only. Commercial entities need to obtain a research use license from Brookhaven National Labs prior to use of this Product. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
产品信息
Components
• 1 µg pTriEx-6 3C/LIC Vector
• 8 µl 3C/LIC β-gal Control Insert
• 25 U T4 DNA Polymerase (LIC-qualified)
• 50 µl 10X T4 DNA Polymerase Buffer
• 100 µl 100 mM DTT
• 40 µl 25 mM dATP
• 50 µl 25 mM EDTA
• 1.5 ml Nuclease-free Water
• 22 × 50 µl NovaBlue GigaSingles™ Competent Cells
• 2 × 0.2 ml Origami™ B(DE3)pLacI Competent Cells
• 5 × 2 ml SOC Medium
• 10 µl Test Plasmid (ampicillin resistant)
商店和运输信息
Storage ≤ -70°C
Ship Dry Ice Only
Multiple Toxicity Values, refer to MSDS |