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Tag•off™ High Activity rEK
Tag-Off High Activity Enterokinase
Increased cleavage rate of fusion proteins
Tag•off™ High Activity rEK is a highly purified preparation of the catalytic subunit of recombinant human enterokinase that recognizes the identical cleavage site as the native enzyme, AspAspAspAspLys↓ (Gasparian 2003). The Tag•off High Activity rEK substrate binding site (ArgArgArgLys) differs from the bovine enterokinase site (LysArgArgLys) by a single amino acid, which is believed to account for its higher substrate affinity and the resulting higher specific activity. The preparation has been functionally tested for activity with thioredoxin/human epidermal growth factor and thioredoxin/ human IL-13 fusion proteins (Gasparian). The Tag•off High Activity rEK as a highly purified (99% by Coomassie staining) enzyme solution and includes rEK 1X Dilution/Storage Buffer, 10X Cleavage Buffer, and a Cleavage Control Protein.
产品信息
Unit definition One unit is defined as the amount of enzyme needed to cleave 50 µg of fusion protein in 16 hours at 23°C in a buffer containing 20 mM Tris-HCl, 50 mM NaCl, and 2 mM CaCl2, pH 7.4.
Components
• 50 U Tag•Off™ High-activity rEK
• 10 µg Cleavage Control Protein
• 2 ml 1X rEK Dilution/Storage Buffer
• 1 ml 10X rEK Cleavage Buffer
商店和运输信息
Storage -20°C
Ship Shipped with Blue Ice or with Dry Ice
Multiple Toxicity Values, refer to MSDS |