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Rosetta™ 2(DE3)pLacI Competent Cells
Expression host strains for pETBlue™ and pTriEx™ vectors are lDE3 lysogens that also carry the compatible pLacI plasmid, encoding the lac repressor. The pLacI plasmid confers chloramphenicol resistance and provides enough lac repressor to inhibit transcription from the T7lac promoter in the absence of inducer. The NovaBlue host strain is recommended for initial cloning with pETBlue and pTriEx vectors due to its superior transformation efficiency and high yields of high-quality plasmid DNA.
Note that these strains are not recommended for use with pET, pACYCDuet™, pCDF, pRSF, pCOLADuet™ or pETcoco™ vectors.
.Rosetta™ 2 strains are BL21 derivatives designed to alleviate codon bias when expressing heterologous proteins in E. coli. The cells carry a chloramphenicol-resistant plasmid with the tRNA genes that decode seven rare codons (AGA, AGG, AUA, CUA, GGA, CCC, and CGG) to improve the yield of full-length proteins. The tRNA genes are driven by their native promoters. In Rosetta 2(DE3)pLacI, the rare tRNA genes are present on the same plasmid that carries the lac repressor gene.
DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG. Genotype: F–ompT hsdSB (rB– mB–) gal dcm (DE3) pLacIRARE2 (CamR)
产品信息
Components
0.4 ml 1 ml Component
• 2 × 0.2 ml 5 × 0.2 ml Rosetta 2(DE3)pLacI Competent Cells
• 2 × 2 ml 4 × 2 ml SOC Medium
• 2 ng 2 ng Test Plasmid
Guaranteed efficiency 2 × 106 cfu/µg
商店和运输信息
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage ≤ -70°C
Ship Dry Ice Only
Multiple Toxicity Values, refer to MSDS
Licence Brookhaven This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license. |