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Rosetta™ 2(DE3)pLysS Singles™ Competent Cells
Rosetta™ 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains supply tRNAs for 7 rare codones (AGA, AGG, AUA, CUA, GGA, CCC, and CGG) on a compatible chloramphenicol-resistant plasmid. The tRNA genes are driven by their native promoters.
DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability. Genotype: F-ompT hsdSB(rB- mB-) gal dcm (DE3) pLysSRARE2 (CamR)
产品信息
Components
• 11 rxn or 22 rxn Rosetta 2(DE3)pLysS Competent Cells
• 2 × 2 ml or 4 × 2 ml SOC Medium
• 10 µl Test Plasmid
Features and benefits • Guaranteed transformation efficiencies:
> 1.5 × 108 cfu/µg (NovaBlue and NovaBlueT1R)
> 2 × 106 cfu/µg [BL21(DE3), BL21(DE3)pLysS, Origami™(DE3), Origami 2(DE3), Rosetta™(DE3), Rosetta 2(DE3)pLysS]
• Provided as frozen single-use aliquots in 11- reaction or 22-reaction kits
• Includes Test Plasmid and SOC Medium
• Selection of popular host strains: NovaBlue for high efficiency cloning, and λDE3 lysogens for protein expression with pET vectors
• Prepared using an optimized chemical method
Guaranteed efficiency > 2 × 106 cfu/mg
商店和运输信息
Storage ≤ -70°C
Ship Dry Ice Only
Multiple Toxicity Values, refer to MSDS
Licence Brookhaven This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license. |