ROSETTA 2(DE3) COMPETENT CELLS

Rosetta™ 2(DE3) Competent Cells
Rosetta™ 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains supply tRNAs for 7 rare codones (AGA, AGG, AUA, CUA, GGA, CCC, and CGG) on a compatible chloramphenicol-resistant plasmid. The tRNA genes are driven by their native promoters.
DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG. Genotype: F-ompT hsdSB(rB- mB-) gal dcm (DE3) pRARE2 (CamR)
相关产品
71402  Rosetta™ 2 Competent Cells
71403  Rosetta™ 2(DE3)pLysS Competent Cells
71405  Rosetta™ 2 Competent Cell Set
产品信息
Components 
• 2 × 200 µl or 5 × 200 µl Rosetta 2(DE3) Competent Cells
• 2 × 2 ml or 4 × 2 ml SOC Medium
• 10 µl Test Plasmid
Guaranteed efficiency  >2 × 106 cfu/µg
商店和运输信息
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage  ≤ -70°C
Ship  Dry Ice Only
Multiple Toxicity Values, refer to MSDS
Licence Brookhaven  This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.