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pET-46 Ek/LIC Vector Kit
The pET-46 Ek/LIC Vector is a LIC-compatible derivative of pET-45b(+). The plasmid contains a strong T7lac promoter and an amino-terminal His•Tag® coding sequence immediately followed by an Ek/LIC cloning site designed to allow the generation of fusion proteins with minimal vector-encoded sequence. An optional C-terminal S•Tag™ coding sequence is compatible with purification, detection, and quantification (1). This plasmid is compatible with the LIC Duet™ Adaptors for coexpression of two proteins as well as with pCDF-2 and pRSF-2 Ek/LIC vectors for coexpression of up to six proteins when using the LIC Duet Adaptors.
The pET System allows for expression of target genes under control of the T7 promoter and is covered by US Patent 5,693,489. This patent is licensed by EMD Millipore Corporation from Brookhaven National Labs. Commercial entities need to obtain a license from Brookhaven National Labs prior to use of this Product. Ccommercial use of this Product also requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
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产品信息
Components
• 1 µg Ek/LIC Vector
• 25 units T4 DNA Polymerase, LIC-qualified
• 50 µl 25 mM EDTA
• 40 µl 25 mM dATP
• 8 µl Ek/LIC β-gal Control Insert
• 100 µl 100 mM DTT
• 50 µl 10X T4 DNA Polymerase Buffer
• 1.5 ml Nuclease-free Water
• 5 × 2 ml SOC Medium
• 10 µl Test Plasmid
• 0.2 ml BL21(DE3)pLysS Competent Cells
• 0.2 ml BL21(DE3) Competent Cells
• 22 × 50 µl NovaBlue GigaSingles™ Competent Cells
商店和运输信息
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage ≤ -70°C
Ship Dry Ice Only
Multiple Toxicity Values, refer to MSDS |