|
SpinPrep™ PCR Clean-up Kit
Rapid purification of PCR products for downstream procedures
The SpinPrep™ PCR Clean-Up Kit is designed for the rapid purification of DNA amplified in PCR. The 10-minute procedure involves addition of a binding buffer followed by adsorption of the DNA to a silica membrane in a spin column format. Following a wash step, the DNA is eluted in low-salt buffer. This kit removes DNA polymerases, dNTPs, salts, and > 99% of primers so that they do not interfere with downstream applications such as cloning, sequencing, or labeling. PCR products from 100 bp to > 12,000 bp can be cleaned up, with recoveries of amplified DNA in the range of 60-90% under standard conditions.
Column binding capacity: up to 6 µg
PCR volume: 100 µl/rxn
Typical recovery: 60–90%
Size range: 100 bp to > 12,000 bp
Time required: < 10 min
产品信息
Components
• 82 ml SpinPrep Bind Buffer
• 27 ml SpinPrep Wash Buffer
• 10 ml SpinPrep Elute Buffer
• 100 SpinPrep Filters
• 100 Receiver Tubes
• 100 Eluate Receiver Tubes
商店和运输信息
Storage +15°C to +30°C
Ship Shipped at Ambient Temperature or with Blue Ice
Multiple Toxicity Values, refer to MSDS |