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BugBuster® GST•Bind™ Purification Kit
Affinity purification of GST fusion proteins
The BugBuster® GST•Bind™ Purification Kit combines the GST•Bind Resin, GST•Bind Buffer Kit reagents and BugBuster Protein Extraction Reagent for convenient preparation
of soluble cell extracts and affinity purification of GST•Tag™ fusion proteins. BugBuster Protein Extraction Reagent is formulated for the gentle disruption of the
cell wall of E. coli, resulting in the liberation of soluble protein. Cells are harvested by centrifugation as usual, followed by suspension in BugBuster Reagent.
During a brief incubation, soluble proteins are released. The extract is clarified by centrifugation, which removes cell debris and insoluble proteins. The clarified
extract is ready to apply to GST•Bind Resin.
产品信息
Components
• 2 x 100 ml BugBuster Protein Extraction Reagent
• 10,000 U Benzonase Nuclease, purity > 90%
• 10 ml GST•Bind Resin
• pkg/4 Chromatography Columns
• 2 x 100 ml 10X GST Bind/Wash Buffer
• 40 ml 10X Glutathione Reconstitution Buffer
• 1 g Glutathione, Reduced
商店和运输信息
Storage Multiple storage requirements
Ship Blue Ice Only
Multiple Toxicity Values, refer to MSDS
安全标示
S Phrase S: 26
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
R Phrase R: 36/38
Irritating to eyes and skin. |