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Tuner™ Competent Cell Set
Tuner™ strains are lacZY deletion mutants of BL21 and enable adjustable levels of protein expression throughout all cells in a culture. The lac permease (lacY) mutation allows uniform entry of IPTG into all cells in the population, which produces a concentration-dependent, homogeneous level of induction. By adjusting the concentration of IPTG, expression can be regulated from very low levels up to the robust, fully induced levels commonly associated with pET vectors. Lower-level expression may enhance the solubility and activity of difficult target proteins.
DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability. Genotypes:
Tuner
F–ompT hsdSB (rB– mB–) gal dcm lacY1
Tuner(DE3)
F–ompT hsdSB (rB– mB–) gal dcm lacY1(DE3)
Tuner(DE3)pLysS
F–ompT hsdSB (rB– mB–) gal dcm lacY1(DE3) pLysS (CamR)
相关产品
70622 Tuner™ Competent Cells
70623 Tuner™(DE3) Competent Cells
70624 Tuner™(DE3)pLysS Competent Cells
产品信息
Avoid freeze/thaw 是的
Components
• 2 x 0.2 ml Tuner Competent Cells
• 2 x 0.2 ml Tuner(DE3) Competent Cells
• 2 x 0.2 ml Tuner(DE3)pLysS Competent Cells
• 4 x 2 ml SOC Medium
• 2 ng Test Plasmid
商店和运输信息
Storage ≤ -70°C
Ship Dry Ice Only
Multiple Toxicity Values, refer to MSDS
Licence Brookhaven This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license. |