pT7Blue-2 Blunt Vector (linearized) This product has been discontinued. The pT7Blue-2 vector features the pUC19 backbone (including high-copy number origin of replication and lac sequences), f1 origin of replication and modified multiple cloning region designed for optimal expression of cloned inserts by in vitro translation. The multiple cloning region contains a T7 promoter upstream of the Xenopus β-globin 5′ UTR translation enhancer and S•Tag™ sequences, followed by an enterokinase cleavage site and extensive region of cloning sites. An SP6 promoter is placed within the multiple cloning sites to allow the synthesis of essentially unfused RNA from DNA inserted downstream. 相关产品 70185 Introductory pT7Blue-2 Perfectly Blunt® Cloning Kit 70190 pT7Blue-2 Perfectly Blunt® Cloning Kit 产品信息 Avoid freeze/thaw 是的 Components 20 rxn 40 rxn • 2 × 0.5 µg 4 × 0.5 µg Blunt Vector • 10 µl 10 µl Positive Control Insert • 100 µl 2 × 100 µl End Conversion Mix 商店和运输信息 Storage ≤ -70°C Ship Dry Ice Only Standard Handling