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pT7Blue Blunt Vector (linearized)
The pT7Blue vector features the pUC19 backbone (including high-copy number origin of replication and lac sequences), a T7 promoter, f1 origin of replication, and modified multiple cloning region. The multiple cloning region contains an EcoR V site used for T-cloning flanked by an Nde I site, which allows inserts to be conveniently subcloned into the Nde I site of many pET vectors.
相关产品
70183 Introductory pT7Blue Perfectly Blunt® Cloning Kit
70189 pT7Blue Perfectly Blunt® Cloning Kit
产品信息
Avoid freeze/thaw 是的
Components
20 rxn 40 rxn
• 2 × 0.5 µg 4 × 0.5 µg Blunt Vector
• 10 µl 10 µl Positive Control Insert
• 100 µl 2 × 100 µl End Conversion Mix
Features and benefits
No restriction enzymes or special primers
Compatible with any DNA polymerase
Independent of 3′-dA addition or sequence context of DNA ends
Blue/white screening with seven different vectors, including pETBlue expression vectors
Simple protocol takes less than one hour* from PCR product to plating transformants
* All times listed refer to kits using NovaBlue Singles™ Competent Cells and ampicillin or chloramphenicol selection. Giga kits, which use NovaBlue GigaSingles™ Competent Cells, require one hour for outgrowth.
商店和运输信息
Storage ≤ -70°C
Ship Dry Ice Only
Standard Handling |