70123 Protein Refolding Kit Simple solubilization and refolding of inclusion body proteins The Protein Refolding Kit provides the basic reagents to solubilize and refold recombinant proteins that accumulate as inclusion bodies in E. coli by a patented procedure (Burgess 1996, Frankel 1993). Product information Avoid freeze/thaw Yes Components • 100 ml 10X IB Wash Buffer • 50 ml 10X IB Solubilization Buffer • 2 x 100 ml 50X Dialysis Buffer • 2 x 1.6 ml 1M DTT • 10 ml 30% N-lauroylsarcosine Features and benefits Provides for easy isolation and solubilization of inclusion bodies under mild denaturing conditions. Solubilized fraction is dialyzed against a neutral pH buffer containing a reducing agent to encourage correct disulfide bond formation. A second dialysis step removes excess reducing agent and transfers the protein into the buffer of choice Additional separate components such as NDSBs can be added to increase refolding efficiency of individual proteins. Sufficient buffers are provided to process up to 2 g inclusion bodies. Store and ship information Storage +2°C to +8°C Ship Blue Ice Only Multiple Toxicity Values, refer to MSDS