T7Select® Biopanning Kit
Matched reagents for selecting binders from T7Select libraries
Enrichment of T7 phage displaying target peptides or proteins can easily be accomplished with the T7Select® Biopanning Kit. The kit contains reagents to perform biopanning with target ligands using 96-well microtiter plates or similar solid phases. Biopanning procedures can be tested and optimized using the positive and negative controls provided in the kit. The supplied Positive Control Lysate contains a T7Select415-1 recombinant that displays the S•Tag™ peptide. When combined with the Negative Control Lysate at known ratios, the degree of enrichment of high affinity binding phage can be monitored by a simple plaque lift assay with the provided detection reagents.
Biopanning selection
Biopanning requires both binding and elution conditions that maintain the infectivity of the phage. The stability of the T7 phage particle enables the use of a variety of elution conditions during biopanning. Phage maintain infectivity following treatment with 1% SDS, 5 M NaCl, 4 M urea, 2 M guanidine-HCl, 10 mM EDTA, reducing conditions (up to 100 mM DTT), and a pH range of 4 to 10. The wide range of conditions available for T7Select biopanning expands the range of targets that can be used successfully.
产品信息
Avoid freeze/thaw 是的
Components
• 25 g Blocking Reagent
• 2 x 50 ml 10X TBST Wash Buffer
• 10 ml T7 Elution Buffer
• 0.2 ml BL21 Glycerol Stock
• 0.2 ml BLT5403 Glycerol Stock
• 0.2 ml BLT5615 Glycerol Stock
• 10¹0 pfu T7Select Positive Control Lysate
• 10¹0 pfu T7Select Negative Control Lysate
• 50 µl S-protein HRP Conjugate
• 2 ml T7Select S-protein
• 50 ml SuperSignal® Substrate
• pkg/25 gLOCATOR™ Luminescent Labels
• pkg/25 Development Folders
商店和运输信息
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage Multiple storage requirements
Ship Blue Ice Only
Multiple Toxicity Values, refer to MSDS |