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Biotinylated T7•Tag® Monoclonal Antibody
When used with Novagen's Streptavidin Alkaline Phosphatase Conjugate, Biotinylated T7•Tag Antibody enables a 5-fold increase in detection sensitivity of target proteins on Western blots compared to an anti-IgG conjugate strategy. This form of the antibody also enables specific detection in the presence of other mouse IgGs.
The T7•Tag® Antibody is a mouse monoclonal antibody (IgG2b) directed against the 11 amino acid gene 10 leader peptide expressed by many of the pET translation vectors as well as pSCREEN™ and pRSET vectors. Because the peptide is the natural amino terminal end of the T7 major capsid protein, the antibody also recognizes T7 bacteriophage (e.g., the T7Select™ Vectors).
This antibody is also available as a direct conjugate with alkaline phosphatase or horseradish peroxidase.
Specificity T7•Tag peptide MetAlaSerMetThrGlyGlyGlnGlnMetGly; N-terminal, C-terminal or internal
Species/Isotype Mouse monoclonal IgG2b
Cross-reactivity Negligible with bacterial, insect or mammalian cell lysates
Sensitivity 1–10 ng (Western blot developed with chromogenic substrates)
< 1 ng (developed with chemiluminescent substrates)
Form Stabilized solution of antibody conjugate in 50% glycerol; includes Positive Control Extract containing 31.1 kDa target protein
Working dilution 1:5,000–1:10,000
相关产品
69048 T7•Tag® Antibody HRP Conjugate
69522 T7•Tag® Monoclonal Antibody
69999 T7•Tag® Antibody AP Conjugate
产品信息
Avoid freeze/thaw 是的
Components
• 25 µg (sufficient for 125 Western blots (10 cm × 10 cm)
• Positive Control Extract containing 31.1 kDa target protein
商店和运输信息
Storage -20°C
Ship Shipped with Blue Ice or with Dry Ice
Multiple Toxicity Values, refer to MSDS |