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Perfect Protein™ Western Markers
Accurate size markers detectable on any Western blot
The Perfect Protein™ Western Markers consist of seven recombinant S•Tag™/His•Tag® fusion proteins that can be detected on any Western blot using an S-protein AP or HRP
conjugate. As an additional option, the markers can also be detected with an His•Tag Monoclonal Antibody and an AP or HRP conjugated secondary antibody. The Perfect
Protein Western Markers serve as precise size standards that appear simultaneously on the blot with target proteins, eliminating the uncertainty and imprecision
associated with other methods.
For ease of convenience, please consider the Perfect Protein AP Western Blot Kit (Cat. No. 69965), or the Perfect Protein HRP Western Blot Kit (Cat. No. 69078). For
a version containing prestained proteins for tracking electrophoresis and Western transfer, please refer to our complete listing of Trail Mix™ Western Markers and
Blot Kits.
A.Two parallel blots were incubated with primary antibodies for an expressed protein, then incubated with Anti-Mouse IgG AP Conjugate and processed using
chemiluminescent detection. The S-protein AP Conjugate was included with secondary antibody for the blot on the left. Lanes: 1, 5 µl Perfect Protein Western
Markers; 2, 10 ng induced cell extract; 3, 1 µg uninduced cell extract.
B.Blot contains the Perfect Protein Western Markers plus purified GUS expressed in insect cells incubated with a GUS-specific monoclonal antibody, then incubated
with Anti-Mouse IgG HRP Conjugate and S-protein HRP Conjugate and detected with chemiluminescent HRP substrate. Lanes: 1, 5 µl Perfect Protein Western Markers;
2, 800 ng purified GUS protein.
相关产品
69078 Perfect Protein™ HRP Western Blot Kit
69965 Perfect Protein™ AP Western Blot Kit
产品信息
Form Liquid
Avoid freeze/thaw 是的
Features and benefits • Detect markers simply by adding the S-protein (AP or HRP Conjugate) or His•Tag® Monoclonal Antibody to the same incubation used for sample
detection.
• S-protein conjugate (included in kits) does not interfere with antibodies or streptavidin detection.
• Can be used with colorimetric and chemiluminescent AP or HRP substrates.
• Recombinant, unmodified markers give sharp, accurately sized bands.
• Markers are supplied at the working dilution in gel loading buffer; concentration optimized for Western detection.
• Protein sizes are 15, 25, 35, 50, 75, 100, and 150 kDa.
商店和运输信息
Storage -20°C
Ship Blue Ice Only
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