T4 DNA Ligase Qualified under the most stringent cloning conditions 下载 添加到最爱 产品编号 Qty/Pk 数量 价格 该产品目前无法供应或者不存在. T4 DNA Ligase catalyzes the formation of phosphodiester bonds between 3′-hydroxyl and 5′-phosphate groups of adjacent DNA and RNA nucleotides in blunt-end or cohesive-end configurations. The enzyme joins RNA and DNA strands only in duplex molecules and does not join single-stranded nucleic acids. Novagen T4 DNA Ligase is rigorously tested in a blue-white cloning assay under conditions that maximize exposure of DNA ends to any contaminating nucleases. The enzyme is qualified for any ligation or cloning application. 产品信息 Avoid freeze/thaw 是的 Unit definition 4 Weiss units/µl; one Weiss unit is defined as the amount of enzyme required to catalyze the exchange of 1 nmole of 32P from pyrophosphate into ATP as Norit®-absorbable material in 20 minutes at 37°C. 0.01 Weiss unit is the amount of enzyme required to ligate > 95% of 1 µg bacteriophage λ Hind III fragments at 16°C in 20 minutes. 商店和运输信息 Storage -20°C Ship Shipped with Blue Ice or with Dry Ice Standard Handling