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Human Ig-Primer Set
Amplification of human and mouse immunoglobulin variable regions
The Ig-Primer Sets are designed for amplification of immunoglobulin (Ig) variable region cDNAs from human and mouse sources. Mammalian light- and heavy-chain Igs contain conserved regions adjacent to the hypervariable complementarity defining regions (CDRs). Appropriately designed oligonucleotide primer sets enable these regions to be specifically amplified using PCR (Larrick 1989, Jones 1991), after which the products may be sequenced directly or cloned. This powerful method enables rapid, precise analysis of hybridomas, myeloma cell progression and heterogeneity, idiotype networks, B-cell clonal ontogeny, and phylogenetic relationships.
In practice, total RNA is isolated from a B-cell source, such as peripheral blood, lymph node(s), spleen, or hybridoma, and first-strand cDNA is prepared using reverse transcriptase. The primer sets are then used in PCR to specifically amplify the variable regions of light- and heavy-chain cDNAs.
Please call Technical Service if you need more information regarding individual Ig-Primer Sets.
相关产品
69831 Mouse Ig-Primer Set
产品信息
Avoid freeze/thaw 是的
Components
• 500 pmol Hu IgM VH 3′-1 Primer
• 500 pmol Hu IgG VH 3′-1 Primer
• 500 pmol Hu Ig VH 5′-A Primer
• 500 pmol Hu Ig VH 5′-B Primer
• 500 pmol Hu Ig VH 5′-C Primer
• 500 pmol Hu Igκ VL 3′-1 Primer
• 500 pmol Hu Igκ VL 5′-A Primer
• 500 pmol Hu Igλ VL 3′-1 Primer
• 500 pmol Hu Igλ VL 5′-A Primer
• 10 µl Human Positive Control PCR Template
商店和运输信息
Storage -20°C
Ship Shipped with Blue Ice or with Dry Ice
Standard Handling |