|
69734 λDE3 Lysogenization Kit |LAMBDA DE3 LYSOGENIZATION KIT/兰布达DE 3溶原化试剂盒
Construction of bacterial strains that carry inducible T7 RNA Polymerase
The λDE3 Lysogenization Kit is designed for site-specific integration of λDE3 prophage into an E. coli host cell chromosome, such that the lysogenized host can be used to express target genes cloned in pET vectors. λDE3 is a recombinant phage carrying the cloned gene for T7 RNA polymerase under lacUV5 control. Lysogens are prepared by co-infection with the three provided phages, and can be verified by plating the T7 Tester Phage (a T7 RNA polymerase deletion mutant). This phage is unable to make a plaque on cells that lack T7 RNA polymerase, but makes normal plaques on λDE3 lysogens in the presence of IPTG.The T7 RNA Polymerase Monoclonal Antibody can be used to verify the presence of T7 RNA polymerase in the host strain by Western blot analysis.
Related products
69725 λDE3 Lysogenization Kit plus pLysS & pLysE
Related products for λDE3 Lysogenization Kit
69725 λDE3 Lysogenization Kit plus pLysS & pLysE
Product information
Avoid freeze/thaw Yes
Components • 200 µl λDE3 Phage Lysate
• 200 µl Helper Phage Lysate
• 200 µl Selection Phage Lysate
• 50 µl T7 Tester Phage Lysate
• 200 µl HMS174(DE3) glycerol stock
Store and ship information
Storage ≤ -70°C
Ship Dry Ice Only
Multiple Toxicity Values, refer to MSDS
Licence Brookhaven This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license |