|
HMS174(DE3)pLysS Competent Cells
HMS174 strains provide the recA mutation in a K-12 background. Like BLR, these strains may stabilize certain target genes whose products may cause the loss of the DE3 prophage.
DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability. Genotype: F-recA1 hsdR(rK12- mK12+) (DE3) pLysS (CamR, Rif R)
相关产品
69452 HMS174 Competent Cells
69453 HMS174(DE3) Competent Cells
70234 HMS174 Competent Cell Set
产品信息
Avoid freeze/thaw 是的
Components
0.4 ml 1 ml Component
• 2 × 0.2 ml 5 × 0.2 ml HMS174(DE3)pLysS Competent Cells
• 2 × 2 ml 4 × 2 ml SOC Medium
• 2 ng 2 ng Test Plasmid
Guaranteed efficiency Guaranteed efficiency: > 5 x 106 cfu/µg
商店和运输信息
Storage ≤ -70°C
Ship Dry Ice Only
Multiple Toxicity Values, refer to MSDS
Licence Brookhaven This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license. |