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pET-30 Ek/LIC Vector Kit
The pET-30 Ek/LIC vector is an Ek/LIC version of pET-30b(+). It is designed for cloning and high-level expression of target proteins fused with the His•Tag® and S®Tag™ coding sequences that are cleavable with enterokinase (Ek) protease. The plasmid contains a strong T7lac promoter, an optimized RBS, the coding sequence for the Ek protease cleavage site (AspAspAspAspLys↓), and a multiple cloning site that contains restriction enzyme sites found in many other Novagen expression vectors to facilitate insert transfer. An optional C-terminal His•Tag coding sequence is compatible with purification, detection, and quantification.
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产品信息
Avoid freeze/thaw 是的
Components
• 1 µg Ek/LIC Vector
• 10 µl Ek/LIC Control Insert
• 25 U T4 DNA Polymerase, LIC-qualified
• 50 µl 10X T4 DNA Polymerase Buffer
• 100 µl 100 mM DTT
• 50 µl 25 mM EDTA
• 40 µl 25 mM dATP
• 1.5 ml Nuclease-free Water
• 22 × 50 µl NovaBlue GigaSingles™ Competent Cells
• 0.2 ml BL21(DE3) Competent Cells
• 0.2 ml BL21(DE3)pLysS Competent Cells
• 5 × 2 ml SOC Medium
• 10 µl Test Plasmid
商店和运输信息
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage ≤ -70°C
Ship Dry Ice Only
Multiple Toxicity Values, refer to MSDS |