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Thrombin Cleavage Capture Kit
The Thrombin Cleavage Capture Kit is designed for cleavage of fusion proteins followed by convenient and quantitative removal of thrombin protease. The method is based on the use of Biotinylated Thrombin for digestion and its subsequent removal with Streptavidin Agarose. The kit is suitable for use with any fusion protein that contains a thrombin recognition sequence. A Cleavage Control Protein is included in the kit to monitor performance of cleavage conditions. It is cleaved into 2 fragments, which are easily visualized by SDS-PAGE.
The Cleavage Control Protein is also available separately to monitor performance of either thrombin or enterokinase cleavage conditions. The 48 kDa control protein is cleaved into two proteolytic fragments of 35 kDa and 13 kDa, which are easily visualized by SDS-PAGE.
产品信息
Avoid freeze/thaw 是的
Components
• 50 U Biotinylated Thrombin
• 5 × 1 ml 10X Thrombin Cleavage Buffer
• 2 ml 1X Thrombin Dilution/Storage Buffer
• 2 × 0.4 ml Streptavidin Agarose
• 10 µg Cleavage Control Protein
• pkg/10 Spin Filters, 2 ml capacity
商店和运输信息
Storage Multiple storage requirements
Ship Blue Ice Only
Multiple Toxicity Values, refer to MSDS |