Smoothened Agonist, SAG N-Methyl-N′-(3-pyridinylbenzyl)-N′-(3-chlorobenzo[b]thiophene-2-carbonyl)-1,4-diaminocyclohexane, SAG1.3 A cell-permeable benzothiophene compound that modulates the coupling of Smo with its downstream effector by interacting with the Smo heptahelical domain (KD = 59 nM). Shown to induce Smo internalization. Shown to induce Hedgehog pathway activation (EC50 = ∼ 3 nM in NIH 3T3-derived Shh-LIGHT2 cells) and counteracts Cyclopamine-KAAD (Cat. No. 239804) inhibition of Smo. Reported to act as an activator at low concentrations and as an inhibitor at very high concentrations. A 10 mM (500 µg/86 µl) solution of Smoothened Agonist, SAG (Cat. No. 566661) in H2O is also available. 产品信息 Form Light yellow solid Molar mass 599.0 Protect from Light 是的 Hygrocopic 是的 Packaged under inert gas 是的 Purity ≥98% by HPLC Solubility DMSO or H2O Chemical formula C28H28ClN3OS • 2HCl • 2H2O CAS number 364590-63-6 商店和运输信息 Storage +2°C to +8°C Ship Ambient Temperature Only Standard Handling