PROTEIN KINASE A, CATALYTIC SUBUNIT, BOV

PKA, Catalytic Subunit, Bovine Heart
cAMP-Dependent Protein Kinase Catalytic Subunit, Protein Kinase A, Catalytic Subunit
Native PKA catalytic subunit purified from bovine heart. The catalytic subunit of PKA dissociates from the PKA holoenzyme following binding of cAMP to the PKA regulatory subunits and phosphorylates target proteins at serine and/or threonine within the recognition motif, Arg-Arg-X-Ser*-X. The purified monomeric catalytic subunit does not require cAMP for activation and is suitable for studies of PKA phosphorylation in vitro with purified substrates or following its introduction into intact cells or cell extracts. Note: 25 µg ≥ 500,000 units.
产品信息
Form  Liquid
Formulation  In 350 mM K2HPO4, 100 µM DTT, pH 6.8.
Molar mass  40000
Avoid freeze/thaw  是的
Specific activity  ≥20,000 units/µg protein
Unit definition  One unit is defined as the amount of enzyme required to incorporate 1 pmol phosphate into Kemptide substrate (Cat. No. 05-23-4900) per min at 30°C.
Purity  ≥95% by SDS-PAGE
商店和运输信息
Storage  ≤ -70°C
Ship  Dry Ice Only
Standard Handling