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Protein Phosphatase, Lambda, Recombinant, E. coli
Protein Phosphatase, Lambda
Recombinant λ PPase expressed in E. coli. A 221-amino acid, Mn2+-dependent, protein phosphatase. Hydrolyzes phosphate groups on serine, threonine, tyrosine, or histidine residues. Inhibited by orthovanadate. Note: 1KU = 1,000 units. Note: 1 KU = 1000 units.
产品信息
Form Liquid
Formulation In 100 mM NaCl, 50 mM HEPES, 2 mM DTT, 0.1 mM MnCl2, 0.1 mM EGTA, 50% glycerol, 0.01% BRIJ® 35 Detergent, pH 7.5.
Molar mass 25000
Avoid freeze/thaw 是的
Unit definition One unit is defined as the amount of enzyme that will hydrolyze 1.0 nmol of pNPP per min at 30°C, pH 7.8. 20-200 Units will typically remove >95% of phosphates from serine/threonine residues in 0.1 nmol of protein in 30 min. 100-1000 Units will typically remove >95% of phosphates from tyrosine in 0.1 nmol of protein in 30 min.
Activity ≥400,000 units/ml.
Purity ≥95% by SDS-PAGE
Contaminants Protease activity: none detected
Solubility Further dilute in 50 mM Tris-HCl, pH 7.8, 5 mM DTT, 2 mM MnCl2, and 100 µg/ml BSA.
商店和运输信息
Storage ≤ -70°C
Ship Dry Ice Only
Standard Handling |