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Osteopontin, His•Tag®, Mouse, Recombinant, Mouse
Recombinant, mouse osteopontin fused at the C-terminus to a His•Tag® sequence and expressed in NSO-derived Leu17-Asn294 (Glu99Gly) murine myeloma cells. A secreted, highly acidic, Ca2+-binding, RGD-containing, phosphorylated glycoprotein. Chemotactic for macrophages, smooth muscle cells, endothelial cells, and glial cells. Has been shown to inhibit nitric oxide production and cytotoxicity by activated macrophages. Plays a significant role in inducing granulomas of various origin. OPN is also involved in the migration of embryonic fibroblasts, macrophages, and metastatic cells. M.W. 65,000 and 30,000 under reducing conditions.
产品信息
Form Lyophilized
Formulation Lyophilized from 0.2 µm sterile-filtered PBS, 50 µg BSA/µg rmOPN.
Biological activity ~200 ng immobilized osteopontin/well will mediate the adhesion of ≥25% of the 293 cells plated at 105 cells/well
Purity ≥95% by SDS-PAGE
Contaminants Endotoxins: ≤100 pg/µg rmOPN
Solubility Sterile PBS
商店和运输信息
Storage ≤ -70°C
Ship Ambient Temperature Only
Standard Handling |