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Nitric Oxide Assay Kit, Fluorometric
Assay kit useful for the rapid quantitative measurement of nitric oxide (NO). Displays 50-fold increased sensitivity over the colorimetric nitric oxide assay kit (Cat. No. 482650). The assay is based on the enzymatic conversion of nitrate to nitrite by nitrate reductase, followed by the addition of 2,3-diaminonapthalene (DAN), and NaOH, which converts nitrite to a fluorescent compound. Fluorescence measurements of this compound accurately determine the nitrite (NO2-) concentration (excitation max.: 365 nm; emission max.: 450 nm).Do not use with nitrate- or nitrite-containing tissue culture media such as RPMI.
产品信息
Format 96-well plate
Form 192-288 Tests
Detection method Fluorometric
Assay time 3-5 h
Sample type Aqueous solutions
Kit contains Assay Buffer, Nitrate Reductase, Enzyme Cofactors, Nitrate Standard, Nitrite Standard, DAN Reagent, NaOH, Microtiter Plates, Plate Covers, and a user protocol.
商店和运输信息
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage -20°C
Ship Blue Ice Only
Multiple Toxicity Values, refer to MSDS |